Enzyme-linked circumoval precipitin test (ELCOPT) for the diagnosis of schistosomiasis japonica. | Zheng Simin, Tang Danyun, Shi Gongfen, Gong Lingmei, Zhou Texiong | Journal of Parasitology and Parasitic Diseases |

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Title: Enzyme-linked circumoval precipitin test (ELCOPT) for the diagnosis of schistosomiasis japonica.
Personal Authors: Zheng Simin, Tang Danyun, Shi Gongfen, Gong Lingmei, Zhou Texiong
Author Affiliation: Shanghai Inst. of Schistosomiasis Control & Res., Shanghai, China.
Editors: No editors
Document Title: Journal of Parasitology and Parasitic Diseases

Abstract:

A modified ELCOPT technique using heat-treated, ultrasonicated dry egg antigen of Schistosoma japonicum is reported. The relative sensitivity of the test was compared with that of COPT, ELISA and IHA using sera from 327 parasitologically confirmed cases. The positivity rate of ELCOPT was 96.0%, compared with 94.2% for COPT; the average % of ova with precipitates in ELCOPT was 27.9% compared with 15.7% in COPT (significant difference). The positivity rates of ELISA and IHA were 89.6% and 82.1% respectively. Sera from 43 healthy persons were all negative by ELCOPT. Sera from 3 of 87 children with presumed hepatitis produced positively coloured precipitates; the % of ova with precipitates was 1 to 2%. The microplate ELCOPT technique using heat-treated, ultrasonicated dry egg antigen and the appropriate substrate (0.4% benzidine) may be useful in large scale sero-epidemiological surveys. [From English summary]

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